Time-Resolved Fluorescence in Photodynamic Therapy

Time-Resolved Fluorescence in Photodynamic Therapy

Photodynamic therapy (PDT) has been used clinically for treating various diseases including malignant tumors. The main advantages of PDT over traditional cancer treatments are attributed to the localized effects of the photochemical reactions by selective illumination, which then generate reactive oxygen species and singlet oxygen molecules that lead to cell death. To date, overor under-treatme...

Superficial radially resolved fluorescence and 3D photochemical time-dependent model for photodynamic therapy.

Photodynamic therapy (PDT) dosimetric tools are crucial for treatment planning and noninvasive monitoring by means of fluorescence. Present approaches consider usually a 1D problem, a simple photochemical process, or a spatially homogeneous photosensitizer. In this work, a radially resolved superficial photosensitizer fluorescence and 3D photochemical time-dependent PDT model are presented. The...

Time-resolved fluorescence microscopy.

In fluorescence microscopy, the fluorescence emission can be characterised not only by intensity and position, but also by lifetime, polarization and wavelength. Fluorescence lifetime imaging (FLIM) can report on photophysical events that are difficult or impossible to observe by fluorescence intensity imaging, and time-resolved fluorescence anisotropy imaging (TR-FAIM) can measure the rotation...

Time-resolved fluorescence spectroscopy

Photodynamic Therapy and Simultaneous Fluorescence Imaging

In vivo biological application of upconversion nanoparticles (UCNPs) prefers excitation of 700-850 nm, instead of 980 nm, due to the absorption of water. Recently approach in constructing robust Nd doped UCNPs subject to 808 nm excitation relies on a thick Nd sensitized shell. However, for the very 10 important and popular Förster resonance energy transfer (FRET)-based applications, such as pho...